Investigation of ELISA and PCR for Diagnosis of Infectious Mononucleosis
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Investigation of ELISA and PCR for Diagnosis of Infectious Mononucleosis
Background and Aims: Infectious mononucleosis (IM)is the clinical manifestation of primary infection with Epstein-Barr virus (EBV). Humans are the only known reservoir of EBV. Regarding the problems in diagnosis of the disease, the purpose of this study was to assess Enzyme-linked immunosorbent assay (ELISA) and Nested polymerase chain reaction (PCR) as a diagnostic tool for this disease. Mate...
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Epstein–Barr virus is primarily the cause of acute infectious mononucleosis and can also cause lymphoma and autoimmune diseases. Th17 cells, which are a unique subset of ThCD4+ cells, direct the infection toward inflammation through production of inflammatory cytokine IL-17. In contrast, Treg Foxp3 cells inhibit inflammation through secretion of anti-inflammatory cytokine IL-10, leading to chro...
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INTRODUCTION Infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV) is commonly diagnosed by detection of antibodies in the patient's sera. Differentiation of acute from chronic and differential diagnosis of EBV-induced IM from IM-like syndrome caused by human cytomegalovirus (CMV) is important. The objective of this study was to standardize and use polymerase chain reaction (PCR)...
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Background and Aim: Brucellosis is a zoonotic disease and is one of the major health problems in our country. Considering its nonspecific symptoms, diagnosis of this disease is often made by laboratory methods. The purpose of the present study was to compare the sensitivity and specificity of ELISA and a relatively new modified Rose Bengal test in the diagnosis of brucellosis. Material and Meth...
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Background: Epstein-Bar virus (EBV) causes infectious mononucleosis in more than 90% of children. Rarely, mononucleosis may lead to malignancy. The aim the current study was to introduce a child with mononucleosis and persistent adenopathy. Case report: A 4-year-old male patient presented with complaints of fever, shortness of breath and sore throat. He was treated with antibiotics and prednis...
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